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samplesweremixedwith orange loading dye  (New England Biolabs)


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    Structured Review

    New England Biolabs samplesweremixedwith orange loading dye
    Samplesweremixedwith Orange Loading Dye, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 104 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/orange+gel+loading+dye/Gel+Loading+Dye+Orange/pm41571747-344-0-4
    Average 96 stars, based on 104 article reviews
    samplesweremixedwith orange loading dye - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: miRNA-target complementarity in cnidarians resembles its counterpart in plants
    Article Snippet: For overnight ligation of 3’ adapter, T4 RNA Ligase 2 truncated KQ and Murine RNAse inhibitor were used (New England Biolabs). .. For PCR amplification 18 cycles were used and amplified cDNA was run on 2% CertifiedTM Low Range Ultra Agarose (Bio-Rad) gel stained with orange gel loading dye (New England Biolabs). cDNA in the size of 138-150 nucleotides was cut from gel (corresponding to size-selected sRNAs) and extracted with NucleoSpin Gel and PCR Clean-up (Macherey-Nagel, Germany) with elution volume of 12 μl. .. Final concentrations were measured with QubitTM dsDNA HS (high sensitivity) Assay Kit (Thermo Fisher Scientific).

    Article Title: Characterization of RNA interference in the model cnidarian Nematostella vectensis reveals partial target silencing but lack of small RNA amplification
    Article Snippet: For overnight ligation of 3’ adapter, T4 RNA Ligase 2 truncated KQ and Murine RNase inhibitor were used (New England Biolabs). .. The ligated products were subjected to 13-18 cycles of PCR amplification then Certified Low Range Ultra Agarose (Bio-Rad) stained with orange gel loading dye (New England Biolabs). .. Bands ranging between 137 and 149 nucleotides (corresponding to adapter ligated small RNAs) were cut from gel and purified using NucleoSpin Gel and PCR Clean-up (Macherey-Nagel, Germany) with elution volume of 12 μl.

    Article Title: miRNA-target complementarity in cnidarians resembles its counterpart in plants.
    Article Snippet: For overnight ligation of 3’ adapter, T4 RNA Ligase 2 truncated KQ and Murine RNAse inhibitor were used (New England Biolabs). .. For PCR amplification 18 cycles were used and amplified cDNA was run on 2% CertifiedTM Low Range Ultra Agarose (Bio-Rad) gel stained with orange gel loading dye (New England Biolabs). cDNA in the size of 138-150 nucleotides was cut from gel (corresponding to size-selected sRNAs) and extracted with NucleoSpin Gel and PCR Clean-up (Macherey-Nagel, Germany) with elution volume of 12 μl. .. Final concentrations were measured with QubitTM dsDNA HS (high sensitivity) Assay Kit (Thermo Fisher Scientific).

    Article Title: Characterization of RNA interference in the cnidarian Nematostella vectensis reveals partial target silencing but lack of small RNA amplification
    Article Snippet: For overnight ligation of 3′ adapter, T4 RNA Ligase 2 truncated KQ and Murine RNase inhibitor were used (New England Biolabs). .. The ligated products were subjected to 13–18 cycles of PCR amplification then Certified Low Range Ultra Agarose (Bio-Rad) stained with orange gel loading dye (New England Biolabs). .. Bands ranging between 137 and 149 nts (corresponding to adapter ligated small RNAs) were cut from gel and purified using NucleoSpin Gel and PCR Clean-up (Macherey-Nagel, Germany) with elution volume of 12 μl.

    Amplification:

    Article Title: miRNA-target complementarity in cnidarians resembles its counterpart in plants
    Article Snippet: For overnight ligation of 3’ adapter, T4 RNA Ligase 2 truncated KQ and Murine RNAse inhibitor were used (New England Biolabs). .. For PCR amplification 18 cycles were used and amplified cDNA was run on 2% CertifiedTM Low Range Ultra Agarose (Bio-Rad) gel stained with orange gel loading dye (New England Biolabs). cDNA in the size of 138-150 nucleotides was cut from gel (corresponding to size-selected sRNAs) and extracted with NucleoSpin Gel and PCR Clean-up (Macherey-Nagel, Germany) with elution volume of 12 μl. .. Final concentrations were measured with QubitTM dsDNA HS (high sensitivity) Assay Kit (Thermo Fisher Scientific).

    Article Title: Characterization of RNA interference in the model cnidarian Nematostella vectensis reveals partial target silencing but lack of small RNA amplification
    Article Snippet: For overnight ligation of 3’ adapter, T4 RNA Ligase 2 truncated KQ and Murine RNase inhibitor were used (New England Biolabs). .. The ligated products were subjected to 13-18 cycles of PCR amplification then Certified Low Range Ultra Agarose (Bio-Rad) stained with orange gel loading dye (New England Biolabs). .. Bands ranging between 137 and 149 nucleotides (corresponding to adapter ligated small RNAs) were cut from gel and purified using NucleoSpin Gel and PCR Clean-up (Macherey-Nagel, Germany) with elution volume of 12 μl.

    Article Title: miRNA-target complementarity in cnidarians resembles its counterpart in plants.
    Article Snippet: For overnight ligation of 3’ adapter, T4 RNA Ligase 2 truncated KQ and Murine RNAse inhibitor were used (New England Biolabs). .. For PCR amplification 18 cycles were used and amplified cDNA was run on 2% CertifiedTM Low Range Ultra Agarose (Bio-Rad) gel stained with orange gel loading dye (New England Biolabs). cDNA in the size of 138-150 nucleotides was cut from gel (corresponding to size-selected sRNAs) and extracted with NucleoSpin Gel and PCR Clean-up (Macherey-Nagel, Germany) with elution volume of 12 μl. .. Final concentrations were measured with QubitTM dsDNA HS (high sensitivity) Assay Kit (Thermo Fisher Scientific).

    Article Title: Characterization of RNA interference in the cnidarian Nematostella vectensis reveals partial target silencing but lack of small RNA amplification
    Article Snippet: For overnight ligation of 3′ adapter, T4 RNA Ligase 2 truncated KQ and Murine RNase inhibitor were used (New England Biolabs). .. The ligated products were subjected to 13–18 cycles of PCR amplification then Certified Low Range Ultra Agarose (Bio-Rad) stained with orange gel loading dye (New England Biolabs). .. Bands ranging between 137 and 149 nts (corresponding to adapter ligated small RNAs) were cut from gel and purified using NucleoSpin Gel and PCR Clean-up (Macherey-Nagel, Germany) with elution volume of 12 μl.

    Staining:

    Article Title: miRNA-target complementarity in cnidarians resembles its counterpart in plants
    Article Snippet: For overnight ligation of 3’ adapter, T4 RNA Ligase 2 truncated KQ and Murine RNAse inhibitor were used (New England Biolabs). .. For PCR amplification 18 cycles were used and amplified cDNA was run on 2% CertifiedTM Low Range Ultra Agarose (Bio-Rad) gel stained with orange gel loading dye (New England Biolabs). cDNA in the size of 138-150 nucleotides was cut from gel (corresponding to size-selected sRNAs) and extracted with NucleoSpin Gel and PCR Clean-up (Macherey-Nagel, Germany) with elution volume of 12 μl. .. Final concentrations were measured with QubitTM dsDNA HS (high sensitivity) Assay Kit (Thermo Fisher Scientific).

    Article Title: Characterization of RNA interference in the model cnidarian Nematostella vectensis reveals partial target silencing but lack of small RNA amplification
    Article Snippet: For overnight ligation of 3’ adapter, T4 RNA Ligase 2 truncated KQ and Murine RNase inhibitor were used (New England Biolabs). .. The ligated products were subjected to 13-18 cycles of PCR amplification then Certified Low Range Ultra Agarose (Bio-Rad) stained with orange gel loading dye (New England Biolabs). .. Bands ranging between 137 and 149 nucleotides (corresponding to adapter ligated small RNAs) were cut from gel and purified using NucleoSpin Gel and PCR Clean-up (Macherey-Nagel, Germany) with elution volume of 12 μl.

    Article Title: miRNA-target complementarity in cnidarians resembles its counterpart in plants.
    Article Snippet: For overnight ligation of 3’ adapter, T4 RNA Ligase 2 truncated KQ and Murine RNAse inhibitor were used (New England Biolabs). .. For PCR amplification 18 cycles were used and amplified cDNA was run on 2% CertifiedTM Low Range Ultra Agarose (Bio-Rad) gel stained with orange gel loading dye (New England Biolabs). cDNA in the size of 138-150 nucleotides was cut from gel (corresponding to size-selected sRNAs) and extracted with NucleoSpin Gel and PCR Clean-up (Macherey-Nagel, Germany) with elution volume of 12 μl. .. Final concentrations were measured with QubitTM dsDNA HS (high sensitivity) Assay Kit (Thermo Fisher Scientific).

    Article Title: Characterization of RNA interference in the cnidarian Nematostella vectensis reveals partial target silencing but lack of small RNA amplification
    Article Snippet: For overnight ligation of 3′ adapter, T4 RNA Ligase 2 truncated KQ and Murine RNase inhibitor were used (New England Biolabs). .. The ligated products were subjected to 13–18 cycles of PCR amplification then Certified Low Range Ultra Agarose (Bio-Rad) stained with orange gel loading dye (New England Biolabs). .. Bands ranging between 137 and 149 nts (corresponding to adapter ligated small RNAs) were cut from gel and purified using NucleoSpin Gel and PCR Clean-up (Macherey-Nagel, Germany) with elution volume of 12 μl.

    Incubation:

    Article Title: Two redox-responsive LysR-type transcription factors control the oxidative stress response of Agrobacterium tumefaciens
    Article Snippet: .. After a 20-min incubation at room temperature, the reaction was stopped by adding 6 × Orange Gel Loading Dye (NEB). .. A total of 15 μl per sample was loaded onto a 2.5% agarose gel prepared with 0.5 × Tris-Borate-EDTA (TBE) buffer and electrophoresed at 120 V for 60 min. Gels were stained with GelRed (Biotium) at a 1:3000 dilution for 30 min and imaged using the ChemiDoc MP Imaging System (Bio-Rad).

    Article Title: Two redox-responsive LysR-type transcription factors control the oxidative stress response of Agrobacterium tumefaciens.
    Article Snippet: .. After a 20-min incubation at room temperature, the reaction was stopped by adding 6 × Orange Gel Loading Dye (NEB). .. A total of 15 μl per sample was loaded onto a 2.5% agarose gel prepared with 0.5 × Tris-Borate-EDTA (TBE) buffer and electrophoresed at 120 V for 60 min. Gels were stained with GelRed (Biotium) at a 1:3000 dilution for 30 min and imaged using the ChemiDoc MP Imaging System (Bio-Rad).



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